Journal: Scientific Reports

Article Title: Arrowtail RNA for Ligand Display on Ginger Exosome-like Nanovesicles to Systemic Deliver siRNA for Cancer Suppression

doi: 10.1038/s41598-018-32953-7

Figure Lengend Snippet: Cancer cell targeting and delivery of siRNA by ligand-displaying GDENs. ( A ) Cancer cell targeting capacity was evaluated by flowcytometry showed dose-dependent of FA-arrowtail RNA ratio verse equal amount of GDENs. ( B ) qRT-PCR evaluating in vitro delivery of survivin siRNA to KB cells by FA-arrowtail RNA ligand displayed GDENs. mRNA levels were standardized by PBS group as 1 and shown as mean ± S.D. fold changes of individual groups. Results are presented at n = 3 for each group for one-way ANOVA multiple comparisons, Tukey’s adjusted p of FA-3WJ/GDENs/sur compare to transfection, 3WJ/GDENs/sur and FA-3WJ/GDENs/scramble are >0.9999, 0.9174 and <0.0001(****). ( C ) Cytotoxicity of FA-3WJ/GDENs evaluated by MTT assay expressed as histogram and fit with nonlinear regression curve ( D ). Samples were incubated with cells in quadruplicate for 24 hours before adding MTT dye. Cell viability were standardized by the control group of “Cell Only” as 100%. Equivalent lipofectamin 2000 were used for comparison. N = 4, error bar indicates ± S.D.

Article Snippet: Statistical mean differences were evaluated using unpaired t-test or ANOVA (p value adjusted for multiple comparisons by Tukey’s procedure with GraphPad software, and p < 0.05 was considered statistically significant).

Techniques: Quantitative RT-PCR, In Vitro, Transfection, MTT Assay, Incubation, Control, Comparison